Method for preserving tissue including immersing the tissue in a solution having a cryoprotectant concentration of at least 75% by weight, a cooling step where the tissue is cooled to a temperature between the glass transition temperature of the solution having a cryoprotectant concentration of at least 75% by weight and -20° C., a storage step where the tissue is stored at a temperature between the glass transition temperature of the solution and -20° C., a rewarming step, where the tissue is warmed, and a washing step.
