high purity acid type sophorolipid characterized in that the substantially free of acetic acid ( SL) inclusions. The high purity acid type SL inclusions can be produced in the following manner for example: (i) obtain a pH of crude acid type SL inclusions in the process is adjusted to an acidic region, and (ii-a) wherein step (i) step of obtaining an acid-type SL fraction was subjected obtained acidified crude acid type SL inclusions chromatography, (ii-b) the step acidifying crude acid type SL-containing product obtained in (i) process was allowed to stand in a cold condition, to obtain a gel product produced.酢酸を実質的に含まないことを特徴とする高純度酸型ソホロリピッド(SL)含有物。当該高純度酸型SL含有物は例えば下記の方法で製造できる:(i)粗精製酸型SL含有物のpHを酸性領域に調整する工程、及び(ii-a)前記工程(i)で得られた酸性化粗精製酸型SL含有物をクロマトグラフィーに供して酸型SL画分を取得する工程、(ii-b)前記工程(i)で得られた酸性化粗精製酸型SL含有物を低温条件下に静置し、生じたゲル化物を取得する工程。