(1) step of forming aggregates of uniform stem cells in serum-free medium of the invention include (2) step of suspension-cultured aggregates of homogeneous stem cells in the presence of basement membrane preparation and stem cells It is intended to provide a method of induced to differentiate into neural progenitor cells. [Selection] Figure Figure 1本発明は、無血清培地中で均一な幹細胞の凝集体を形成させる工程(1);及び基底膜標品の存在下で均一な幹細胞の凝集体を浮遊培養させる工程(2)を含む、幹細胞を神経前駆細胞へ分化誘導する方法を提供する。【選択図】図1