The present disclosure relates to methods for measuring affective bandwidth of a subject (e.g., a human) and determining depression determination of the subject. The affective bandwidth measurement may include the following operations. For example, the subject may watch positive neutral and negative pictures for 8-10 seconds, respectively. Information for all fixation points may be obtained using an eye movement tracking device. Pupil diameter sizes of the subject while watching the positive, neutral and negative picture tasks may be calculated, and the affective bandwidth may be calculated. The affective bandwidth may include positive affective bandwidth (positive-neutral), negative affective bandwidth (negative-neutral) and positive-negative affective bandwidth (positive-negative) of the subject.