SPATZ, MARTIN DIEGO LINUS,GOLDBAUM, FERNANDO ALBERTO,ZYLBERMAN, VANESA,CRAIG, PATRICIO OLIVER,GHERSI, GISELLE,PALERMO, MARINA SANDRA,MEJIAS, MARIA PILAR,BENTANCOR, LETICIA VERONICA
申请号：
CA2913702
公开号：
CA2913702A1
申请日:
2014.05.26
申请国别(地区):
CA
年份:
2014
代理人:
摘要：
The present invention relates to chimeric polypeptides useful as immunogens for inducing protective immune responses and neutralizing antibodies against Shiga toxin (Stx) in mammals. More specifically, the present invention relates to chimeric polypeptides comprising a monomer of the homopentameric B subunit of the Shiga 2 toxin fused to the N-terminus of a monomer of Brucella lumazine synthase, and to oligomeric protein complexes formed from said chimeric polypeptides. The invention further relates to polynucleotides and vectors encoding said chimeric polypeptides, to transgenic cells comprising said polynucleotides and vectors, and to pharmaceutical compositions, such as a vaccine, comprising said chimeric polypeptides and chimeric polynucleotides. Also within the scope of the invention are antibodies which bind to the chimeric polypeptides of the invention, a method for obtaining antibodies which bind specifically to the subunit B of the Shiga 2 toxin and methods of lowering the bacterial load of enterohemorrhagic Escherichia coli in mammals, which can be used for prevention of inter alia, hemolytic-ureic syndrome (HUS).
