In preparing device adipose tissue for transplantation from lobular fat that has been extracted by such as liposuction, oil components, cell debris with dimensions a non-uniform and fluid components including a sterile solution and blood components and / or the fat cells and is composed of a solid component comprising a macroscopic aggregates of one or more cells, provided with (101) cleaning chamber for cleaning materials that are liposuction least wash separation vessel one said apparatus (1) and a, and outlet (102) the inlet (103) is provided with said container (1), it is the liposuction material that is the liposuction enters (101) within said cleaning chamber from (102) the inlet At least a portion of the material has, is configured to fluid component exits (101) the cleaning chamber through a (103) said outlet particular, the wash chamber (101) has a means for emulsifying fluid components . A method of performing a biological or stimulation / treatment volume defects of the face and body, and improvement of the basic nutrients of skin with adipose tissue obtained by the method and the apparatus, the adipose tissue, cartilage are suitable for use as a cell source cells, such as adipocytes and osteocytes. [Selection] Figure Figure 1脂肪吸引などによって抽出された小葉脂肪から移植用の脂肪組織を準備する装置において、前記脂肪は、油成分、血液成分及び/又は滅菌溶液を含む流体成分並びに不均一な寸法を有する細胞片、細胞及び一以上の細胞の巨視的凝集体を含む固体成分から構成され、前記装置は、脂肪吸引された物質を洗浄するための洗浄チャンバ(101)が設けられた少なくとも一つの洗浄分離容器(1)を有し、前記容器(1)には入口(102)及び出口(103)が設けられ、前記脂肪吸引された物質が前記入口(102)から前記洗浄チャンバ(101)内に入り前記脂肪吸引された物質の少なくとも一部、特に流体成分、が前記出口(103)を通って前記洗浄チャンバ(101)から出るよう構成され、前記洗浄チャンバ(101)は流体成分を乳化させる手段を有している。前記装置及び方法を用いて得られた脂肪組織を用いて体及び顔面の体積欠陥の治療、皮膚の基礎栄養の改善及び/又は生物学的刺激を行う方法であって、前記脂肪組織は、軟骨細胞、骨細胞及び脂肪細胞などの細胞源として使用するのに適する。【選択図】図1