An integral vacuum blood collection apparatus with an indwelling catheter comprises a puncture needle tube (1) with an operation handle, a soft extension pipe (13), a blood collection needle tube (12), a blood sample collection pipe (2), and a blood collection needle seat (3). The blood collection needle seat (3) has a cylindrical cavity (31), a tail end of the cylindrical cavity (31) is open, and the blood collection needle tube (12) is fixed at a front wall of the tail end and extends backwards into the cylindrical cavity (31). A pipe plug of the blood sample collection pipe (2) is inserted in the cylindrical cavity (31) in a pluggable and unpluggable manner, and the gap thickness between an front end surface of the blood sample collection pipe (2) and a front end wall of the cylindrical cavity (31) is less than the thickness of a sealing layer (21) of a rear end wall of the pipe plug of the blood sample collection pipe (2), wherein the sealing layer (21) can be punctured by a rear end of the blood collection needle tube. A connection structure matching with the pipe plug of the blood sample collection pipe (2) is arranged in the cylindrical cavity (31), and the cylindrical cavity (31) coordinates with the pipe plug of the blood sample collection pipe (2) to form a firm connection relationship, and the blood sample collection pipe (2) is brought into or taken out of the cylindrical cavity (31) through the structure, so that the blood sample collection pipe (2) inserted in the cylindrical cavity (31) does not fall off when the blood sample collection pipe (2) is placed upright. The puncture needle tube (1) is an indwelling needle, a core of the indwelling needle pipe, a core of the soft extension pipe (13), a core of the blood collection needle tube (12), and a chamber of the blood sample collection pipe (2) whose sealing layer (21) is punctured form a blood channel, and after blood is collected, the tube can still be indwelled to carry out transfusion.La présente