Disclosed is an in vitro human vacuum blood sampling apparatus, in particular a needle cylinder type vacuum blood sampler with a slidable puncturing needle seat, comprising a blood vessel-puncturing needle tube (11), a puncturing needle seat (12), a blood-sampling needle tube (21), a blood-sampling needle seat (22), a soft connecting tube (3) and a cylinder-shaped needle cylinder (5), wherein the soft connecting tube (3) is in communication with the puncturing needle seat (12) and the blood-sampling needle seat (22), a vacuum blood-sampling tube (4) can be inserted into a cylinder-shaped empty cavity at the back of the needle cylinder (5), and the main points of the composition thereof lie in: the blood-sampling needle tube (21), the blood-sampling needle seat (22), and a blood-sampling slidable arm and a blood-sampling pusher (25) which form a blood-sampling component, which blood-sampling component is installed on the needle cylinder (5) in a manner of capable of sliding relative to the needle cylinder (5), the blood-sampling needle tube (21) and the blood-sampling needle seat (22) are provided in the inner cavity of the needle cylinder (5), the blood-sampling slidable arm is connected inside and outside to the blood-sampling needle seat (22) and the blood-sampling pusher (25) through a blood-sampling chute (52) on the side wall of the needle cylinder, the blood-sampling pusher (25) is provided outside the needle cylinder (5), and the blood-sampling chute (52) extends axially along the needle cylinder (5). Compared with the prior art, the present invention has the following advantages: a large operating force, can easily apply force, is stable in operation, reasonable in structure, the manufacturability of the assembly is good and is capable of preventing blood from backflowing.Linvention concerne un appareil de prélèvement in vitro de sang humain sous vide, en particulier une aiguille de prélèvement sanguin sous vide de type cylindre à aiguille présentant un sièg
