A controller for use with a hollow endoscopic needle for collecting tissue, cell and/or fluid specimens from a patient that includes an outer body enclosing a slidably retaining a spring loaded plunger, a click and release plunger holder, a vacuum port, and a hollow needle attached to the plunger that is slidably retained in an outer tube or sheath. The controller permits a surgeon to positively control the needle and penetrate tissue, to pulse the needle, and to quickly and positively return the plunger and its needle back to its original un-pushed position. A vacuum or fluid pathway is defined between proximal and distal seals and along the needles exterior and the interior of the outer tube or sheath and leads to a distal end of the hollow needle. A stylet can be slidably retained within the hollow needle, and can include a sensor to permit navigation.
