INSTITUTE FOR RESEARCH IN BIOMEDICINE (IRB);YALE UNIVERSITY;REGENERON PHARMACEUTICALS INC.
发明人:
Murphy, Andrew,Flavell, Richard,Manz, Markus,Rongvaux, Anthony,Strowig, Till,Willinger, Tim,Stevens, Sean,Yancopoulos, George
申请号：
NZ74195113
公开号：
NZ741951A
申请日:
2013.09.06
申请国别(地区):
NZ
年份:
2020
代理人:
摘要：
The invention relates generally to a method for in vivo collection of a human immune mediator, comprising collecting the immune mediator from a genetically modified mouse, wherein the genetically modified mouse comprises in its genome a nucleic acid encoding human M-CSF, a nucleic acid encoding human IL-3, a nucleic acid encoding human GM-CSF, a nucleic acid encoding human SIRPA and a nucleic acid encoding human TPO, wherein each of the nucleic acids encoding human M- CSF, human IL-3, human GM-CSF, human SIRPA and human TPO is operably linked to a promoter, wherein the mouse expresses human M-CSF polypeptide, human IL-3 polypeptide, human GM-CSF polypeptide, human SIRPA polypeptide and human TPO polypeptide, wherein the mouse comprises a recombination activating gene 2 (Rag2) gene knock-out and an IL2 receptor gamma chain (IL2rg) gene knock-out, and wherein the mouse comprises human hematopoietic cells.
