A probiotic composition essentially comprises 15 to 20 wt % of milk powder, 25 to 30 wt % of corn starch, 8 to 15 wt % of modified starch (capsul), 10 to 15 wt % of ethylcellulose, 5 to 15 wt % of bacterial broth, and 10 to 15 wt % of talc. The probiotic composition is microencapsulated to form a plurality of microencapsule coated with an acid-resistant enteric coating for improving the enteric acid-resistance, the probiotic survival rate, the antimicrobial property, the stability, the moisture-proof property, and the mobility of the probiotic composition preventing from coagulation in a moist environment and for being used as an additive applied to livestock feed.本發明係為一種具有腸溶性耐胃酸包覆之益生菌組成份,主要包括:奶粉(milk powder)(15~20 wt%)、玉米澱粉(corn starch)(25~30 wt%)、改質澱粉(modified starch)(8~15wt%)、乙基纖維素(ethyl cellulose)(10~15 wt%)、菌體(bacterial broth)(5~10wt%)及滑石粉(talc)(10~15 wt%)所組成,該微膠囊化腸溶性生菌劑,係經過包覆材的包覆後,使本發明的產品具有可耐酸腸溶性及生菌劑存活力提高的最佳效果,又具有產品的抑菌性與穩定性,並且具有抗濕性及高滑動性不會因潮濕環境而結塊,可應用於禽畜飼養之食料添加使用。