The present invention provides a therapeutic composition comprising:(i) Trolox, Na+, K+, Ca2+, Mg2+, Cl−, H2PO4−, HEPES, lactobionate, sucrose, mannitol, glucose, dextran-40, adenosine, glutathione; and(ii) stem cells or progenitor cells,wherein the composition does not comprise a dipolar aprotic solvent, in particular DMSO.The present invention also relates to methods of formulating said composition for cryopreservation and subsequent direct administration to a patient, and medicaments comprising said composition.