Provided is a tissue regeneration material release-inducing cell therapeutic composition including a medium for cryopreservation of an animal cell which contains a protein, a sugar, a buffer, and a basal medium and does not contain DMSO, glycerol, and serum. The cell therapeutic composition of the present invention is characterized in that both an animal cell and the medium for cryopreservation of an animal cell have tissue regeneration efficacy, as a tissue regeneration accelerating material within cells is released into the medium for cryopreservation through freezing and thawing processes of the cells. In addition, the cell therapeutic composition of the present invention may be directly cryopreserved in a vial, ampoule or pre-filled syringe, thereby being highly convenient for use, and does not use a cryopreservative agent and serum and thus may be directly applied to a lesion without a separate washing process.
