Vincent Craig Bond,Michael Powell,Ming Bo Huang,Syed Ali
申请号：
US13327244
公开号：
US09777042B2
申请日:
2011.12.15
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest isolating exosomes from the exosome-producing cell culture and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.
