SEYMOUR GRAHAM BARRON,CHAPMAN NATALIE HELENE,BAXTER CHARLES JAMES,PUDDEPHAT IAN
申请号：
NZ70387910
公开号：
NZ703879A
申请日:
2010.10.15
申请国别(地区):
NZ
年份:
2016
代理人:
摘要：
Disclosed is a tomato fruit of a cultivated tomato plant with increased fruit firmness in the outer pericarp at the harvesting stage linked to at least one quantitative trait locus (OTL) in the cultivated tomato plant producing said tomato fruit, wherein said firmness is from between 1.2 to 2.0 times greater than that of fruit from a control tomato plant which does not have said at least one OTL, wherein the harvesting stage is the mature green stage, and wherein said plant can be characterised by: a) the at least one OTL is linked to at least one DNA marker selected from the group consisting of NT3853, NT3907, TG14, HOX7A, CT277, HB2600, TG353, Lm0127, Lm1650, LE5100 and LE5200 and/or b) the at least one OTL is complementary to the corresponding OTL in Solanum pennellii lines IL2-3 and IL2-4 deposited under accession numbers LA4038 and LA4039, respectively, wherein said at least one OTL in LA4038 and LA4039 is linked to at least one DNA marker selected from the group consisting of NT3853, NT3907, TG14, HOX7A, CT277, HB2600, TG353, Lm0127, Lm1650, LE5100 and LE5200 with the proviso that lines IL2-3 and IL2-4 are excluded. Also disclosed is a method for detecting a QTL linked to significantly increased fruit firmness in fruit from a cultivated tomato plant compared to a control tomato plant comprising the steps of a) crossing a donor tomato plant with a recipient tomato plant to provide offspring cultivated tomato plants b) quantitatively determining the firmness in the fruit of said offspring plants c) establishing a genetic linkage map that links the observed increased fruit firmness to the presence of at least one DNA marker from said donor plant in said offspring plants and d) assigning to a QTL the DNA markers on said map that are linked to significantly increased fruit firmness, wherein at least one QTL is as defined in the complete specification.
