The present invention provides a synergistic composition of a nematicide comprising of combination of chalcones, chalcone 17 and chalcone 25, or chalcone 17 and chalcone 30 with potent nematicidal and nematostatic properties. The chalcones individually show 100 % nematicidal activity at a concentration as high as 10-2 - 10-3 M, whereas, the combination of chalcone 17 and chalcone 25, or chalcone 17 and chalcone 30, show 100 % nematicidal activity at concentration as low as 10-4 - 10-6 M bringing a synergistic effect, wherein the ratio of the chalcones is 1:1. These combinations of the chalcones also show potent nematostatic activity compared to each chalcone individually. This synergistic nematicidal composition is environmentally safe and non-toxic to humans and animals.