The present invention provides a synergistic composition of a nematicide comprising of combination of chalcones, chalcone 17 and chalcone 25, or chalcone 17 and chalcone 30 with potent nematicidal and nematostatic properties. The chalcones individually show 100 pcnt nematicidal activity at a concentration as high as 10-2 - 10-3 M, whereas, the combination of chalcone 17 and chalcone 25, or chalcone 17 and chalcone 30, show 100 pcnt nematicidal activity at concentration as low as 10-4 - 10-6 M bringing a synergistic effect, wherein the ratio of the chalcones is 1:1. These combinations of the chalcones also show potent nematostatic activity compared to each chalcone individually. This synergistic nematicidal composition is environmentally safe and non-toxic to humans and animals.