BEHRENS, Sven-Erik,GURSINSKY, Torsten,SCHUCK, Jana
申请号：
WO2018DE00194
公开号：
WO2019001602(A1)
申请日:
2018.06.22
申请国别(地区):
世界知识产权组织国际局
年份:
2019
代理人:
摘要：
Disclosed are methods for the identification and production of highly efficient ("small interfering RNAs") in cytoplasmic extracts of Nicotiana tabacum. The system allows the reconstitution of antiviral RNA-silencing in vivo. More particularly, all DCL (Dicer-like proteins) and AGO (Argonaute) proteins are active in the extracts described. Such siRNAs thus provided are used in gene regulation in target organisms, inter alia in the improvement of pathogen resistance.
