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一种细胞低温保存液、海藻酸钠凝胶、细胞凝胶、制备方法、使用方法及其应用
专利权人:
成都远山原力生物科技有限公司;CHENGDU YUANSHAN BIOTECHNOLOGY CO. LTD
发明人:
YANG, Yuan,杨远,YUAN, Xiang,袁祥,HAN, Fei,韩飞,ZHANG, Zhongwei,张中伟,PAN, Hongwei,潘红伟
申请号:
CNCN2018/109187
公开号:
WO2020/062282A1
申请日:
2018.09.30
申请国别(地区):
CN
年份:
2020
代理人:
摘要:
The present invention relates to the technical field of preservation, regeneration and repair of cells, and disclosed thereby is a low-temperature preservation solution for cells, mainly prepared from the following components in percent by mass: 0.8%-1.5% of mannitol, 2%-3% of human serum albumin, 3%-7% of low-molecular dextran, 0.1-0.3% of glutathione, 0.5%-2% of epigallocatechin gallate, 3%-5% of adenosine, 0.5%-1% of glucose, 0.8% of NaCl, 0.2% of KCl, 0.1% of CaCl2 and the balance of water. Also provided by the present invention is a sodium alginate gel, comprising sodium alginate, calcium chloride, hyaluronic acid and the low-temperature preservation solution for cells, and also provided thereby are a cell gel and a preparation method therefor as well as the application of the low-temperature preservation solution for cells, the sodium alginate gel and the cell gel. The present invention has the advantages of the low-temperature preservation solution for cells being able to maintain cell activity at 2-8°C for a long duration, the sodium alginate gel being able to be used as a temporary short-term preservation material for cells from various sources and brackets for cell tissue engineering, and the cell gel may be used as a medical wet wound dressing and may be used for repairing soft tissues, refractory-healing wound skins and mucosal wound injuries.La présente invention concerne le domaine technique de la conservation, de la régénération et de la réparation de cellules, et concerne ainsi une solution de conservation à basse température pour cellules, qui est principalement préparée à partir des composants suivants exprimés en pourcentage en masse : 0,8 % à 1,5 % de mannitol, 2 % à 3 % de sérumalbumine humaine, 3 % à 7 % de dextrane de faible poids moléculaire, 0,1 à 0,3 % de glutathion, 0,5 % à 2 % de gallate d'épigallocatéchine, 3 % à 5 % d'adénosine, 0,5 % à 1 % de glucose, 0,8 % de NaCl, 0,2 % de KCl, 0,1 % de CaCl2 et le reste d'eau. La présente invention
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来源网址:
http://www.ckcest.cn/home/

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