This invention relates to a low nucleic acid yeast composition and the preparation method thereof. The composition comprises 45-55% of protein, 0.05-1.0% of nucleic acid, 100-5000 ppm of calcium, 30-1000 ppm of iron, and 30-1000 ppm of zinc. The preparation method comprises the steps of: taking the yeast to prepare into aqueous solution of 5-10 mass% adjusting to pH=6.0-10.0 keeping the temperature at 60-100 DEG C for 1-6 hours further, adjusting to pH=5.0-7.0 and separating, washing, and drying the yeast solution, so as to obtain the low nucleic acid yeast composition. This method can easily and effectively reduce the nucleic acid content in the yeast while reserving the nutritional value in the yeast. This invention also relates to a diet composition containing low nucleic acid yeast, which is low-calorie, high nutrition, safe, and effective.本發明係關於一種低核酸酵母組成物及其製備方法。該組成物包含蛋白質45~55%、核酸0.05~1.0%、鈣100~5000ppm、鐵30~1000ppm及鋅30~1000ppm。其製備方法:取酵母配製成5-10%(質量)的水溶液;調整至pH=6.0-10.0;在60-100℃保溫1-6小時;再調整至pH=5.0~7.0;分離、洗滌、乾燥酵母溶液,獲得低核酸酵母組成物。該方法簡便有效降低酵母中核酸含量,並且保留原有酵母中的營養價值。本發明還關於一種包含低核酸酵母的減肥組成物,低熱量、高營養且安全有效。