Given a specific imaging device and systems further described herein, wound characteristics of a wound fluoresce with a unique spectral signature when subjected to excitation light with a known wavelength or range of wavelengths. Images captured therefrom are subject to analyses of pixels thereof, with a plurality of training images having known wound sizes and characteristics marked-up thereon being used to generate training data, which is subsequently used to identify wound characteristics from test images in real time. Wound sizes, boundaries, bacterial presence, and other characteristics may be quantified and graphically represented as an overlay on the original wound image along with documentation related to the wound.