SPECIFIC PRIMERS FOR DIAGNOSING CMV RESISTANCE IN RED PEPPER, AND USES THEREOF
- 专利权人:
- SNU R&DB FOUNDATION
- 发明人:
- KANG, BYOUNG CHEORL,YANG, HEE BUM,KANG, WON HEE
- 申请号:
- KR20080112161
- 公开号:
- KR20100053162(A)
- 申请日:
- 2008.11.12
- 申请国别(地区):
- 韩国
- 年份:
- 2010
- 代理人:
- 摘要:
PURPOSE: A primer set which is specific for CMV resistance diagnosis in Capsicum annuum is provided to find CMV resistance mechanism and to control diseases caused by CMV in various crops. CONSTITUTION: A primer set for diagnosing CMV(Cucumber mosaic virus) resistance in Capsicum annuum comprises an oligonucleotide primer set of sequence numbers 1 and 2 and oliconucleotide primer of sequence numbers 3 and 4. A kit for diagnosing CMV resistance comprises the one or more oligonucleotide set and reagent for amplifying. The reagent contains DNA polymerase, dNTP and buffer. A method for diagnosing CMV resistance in Capsicum annuum comprises: a step of isolating genome DNA form a sample; a step of amplifying the DNA; and a step of detecting amplified product. The detecting of amplified is performed through DNA chip, gel electrophoresis.
- 来源网站:
- 中国工程科技知识中心