PROBLEM TO BE SOLVED: To provide a liposome excellent in temporal stability and a manufacturing method of the same.SOLUTION: A manufacturing method of a liposome encapsulating multiple encapsulated liposomes comprises: a first step which forms a first lipid membrane on a water-oil interface in which oil phase is arranged in contact with water phase a second step which, after forming the encapsulated liposomes, encapsulates a solution including the encapsulated liposomes in a second lipid membrane to form a droplet including the plurality of encapsulated liposomes and a third step which introduces the droplet into the oil phase, and transfers the droplet to the water phase so that outside of the second lipid membrane to be an internal layer lipid membrane is covered with the first lipid membrane to form an external layer lipid membrane.COPYRIGHT: (C)2014,JPO&INPIT【課題】経時安定性に優れたリポソーム及びその製造方法を提供すること。【解決手段】水相に接するかたちで油相を配置した水/油界面に、第1脂質膜を形成する第1工程、被内包リポソームを形成した後、該被内包リポソームを含有した水溶液を第2脂質膜に内包させ、該被内包リポソームを複数含む液滴を作成する第2工程、前記液滴を前記油相に導入し、前記液滴を水相に移行させるとともに、内層脂質膜となる第2脂質膜の外側を第1脂質膜で覆い外層脂質膜を形成する第3工程、を備える。【選択図】図1
