Provided is a vaccine kit against multiple dengue virus serotypes and preparation thereof. The vaccine kit includes a first vaccine and a second vaccine, wherein the first vaccine includes a live attenuated dengue virus, a chimeric live attenuated dengue virus, and the second vaccine comprises multiple types of recombinant flagellin-envelop domain Ⅲ fusion protein. Also provided is an application of a combination, including a live attenuated dengue virus, a chimeric live attenuated dengue virus, an adenoviral vector expressing dengue virus precursor membrane protein and envelope protein, and multiple types of recombinant flagellin-envelop domain Ⅲ fusion protein for preparation of a vaccine kit against multiple dengue virus serotypes.本發明提供一種對抗複數種登革熱病毒血清型之疫苗套組及其製備方法,該疫苗套組包含一第一疫苗與一第二疫苗,其中該第一疫苗包含一登革熱活減毒病毒、一登革熱活減毒嵌合病毒,該第二疫苗包含複數種登革熱病毒血清型之重組第三區域套膜蛋白融合鞭毛蛋白。本發明亦提供該一種包含一登革熱活減毒病毒、一登革熱活減毒嵌合病毒、表現登革熱病毒前膜蛋白及套膜蛋白的腺病毒載體、及一登革熱病毒之重組第三區域套膜蛋白融合鞭毛蛋白之組合用於製備對抗複數種登革熱病毒血清型之疫苗套組之用途。
