VERO CELL LYSIS PROTEINS, METHOD FOR PRODUCTION THEREOF AND KIT FOR DETERMINING HOST CELL PROTEINS FOR VERO CELLS CONTAINING LYSIS PROTEINS
- 专利权人:
- SHANKHAJ ZERUN BIOTEK KO., LTD.
- 发明人:
- KUI DZIJING,LIU JUI,LIU VEJKSU,SHI DZHIN,SHI SONGMING,JAO JUE
- 申请号：
- RU20100143974
- 公开号：
- RU2526131(C2)
- 申请日:
- 2008.08.07
- 申请国别(地区):
- 俄罗斯
- 年份:
- 2014
- 代理人:
- 摘要：
FIELD: chemistry.SUBSTANCE: group of inventions relates to biotechnology and a method of producing proteins that are released during lysis of Vero cells, the method comprising the following steps: culturing and collecting Vero cells; break-down and lysis of Vero cells; purifying proteins released during lysis of Vero cells by hydrophobic chromatography on phenylsepharose 6FF, exclusive chromatography on sepharose, elution and concentration; the released proteins are a mixture of proteins with molecular weight of 11 kDa, 17-34 kDa, 55 kDa and 72-170 kDa. The proteins released during lysis of Vero cell according to the present invention have a wide range of applications. Said proteins can be used to control and analyse quality when producing vaccines using Vero cells with high sensitivity and good reproducibility. The present invention also provides a kit for analysing Vero cell HCP, characterised by high specificity, high sensitivity and good reproducibility.EFFECT: kit can be used to control and analyse qu
- 来源网站：
- 中国工程科技知识中心
