The present invention discloses oligosaccharide peptides with moisturized activity, a manufacturing method thereof and a moisturized formulation thereof. The manufacturing method of the oligosaccharide peptides with moisturized activity comprises the following steps: extracting andprecipitatingmushroom-like plants to obtain polysaccharide peptides and adding hydrolase in the polysaccharide peptides for conducting hydrolysis of the polysaccharide peptides to obtain oligosaccharide peptides. The molecular mass of the oligosaccharide peptides is less than 3000 Dalton. Wherein, the moisturized activity analysis is conducted with the oligosaccharide peptides. The moisturized ratio of the oligosaccharide peptides reaches 85.06% at 25℃. If the moisturized activity analysis is conducted with the oligosaccharide peptides in hot-blast circulating oven at 50℃for 3 hours, the moisturized ratio of the oligosaccharide peptides maintains 10%.本發明係揭露一種具有保濕功能之寡醣肽、其製造方法及其保濕配方。具有保濕功能之寡醣肽之製造方法包含︰將一菇蕈類植物進行萃取及澱析處理以析出一多醣肽,添加一水解酵素於多醣肽中進行水解程序以得到一寡醣肽。寡醣肽之分子質量係小於3000道耳頓(Dalton, Da)。其中,將寡醣肽進行一保濕能力分析試驗,當寡醣肽溶液於25℃下,其保濕能力係達85.06%,而當於50℃下之熱風循環烘箱中,進行試驗3小時後,寡醣肽溶液之保濕能力仍具有10%。S11~S13...步驟