FROZEN CONSERVATIVE SOLUTION FOR DEER SPERM, METHOD FOR FREEZING AND PRESERVING DEER SPERM USING THE SAME, AND METHOD FOR ARTIFICIAL INSEMINATION OF DEER
REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION)
发明人:
KIM, IN CHEOL,LEE, JANG HUI,SON, DONG SU
申请号：
KR20000063870
公开号：
KR20020033843(A)
申请日:
2000.10.30
申请国别(地区):
韩国
年份:
2002
代理人:
摘要：
PURPOSE: A method for artificial insemination of deers by using a frozen conservative solution for deer sperm is provided. By this method, the conception rate of deers is increased because estrus and ovulation are generated in accordance with an artificial insemination time. CONSTITUTION: This frozen conservative solution for deer sperm comprises: a first diluted liquid containing 20%(v/v) egg yolk, 1.6%(w/v) glucose, 1.6%(w/v) fructose, 1.2% Tes-N-tris and 0.5% synthetic clarifiers, based on 100ml distilled water; and a second diluted liquid containing the first diluted liquid and one or more components selected from 16% glycerol, stag serum, pilose antler extracts, bovine serum albumin and heparin. The sperm of deers is diluted with the first diluted liquid in a ratio of 1:5 to 10, additionally diluted to give a final sperm concentration of 1.6 to 3.2x10¬8 sperm/ml and cooled. The cooled sperm is diluted with the second diluted liquid in a ratio of 1:1 and frozen.
