PAOLO DE COPPI,Luca URBANI,Massimo PINZANI,Guiseppe MAZZA,Claire CROWLEY
申请号：
US16617310
公开号：
US20200163326A1
申请日:
2018.06.01
申请国别(地区):
US
年份:
2020
代理人:
摘要：
Methods and materials for the cryopreservation of cellularised scaffolds used for therapeutic or pharmacological testing purposes that provide a cultured scaffold on which cells have been seeded, equilibrate the cellularised scaffold with a cryopreservative composition comprising culture medium and between 5 and 30% of a cryoprotectant such as DMSO, freeze the equilibrated cellularised scaffold by reducing the temperature continuously by about −；1° C./minute to about −；80° C., and store the frozen cellularised scaffold at a temperature of between −；135° C. and −；198° C.
