PURPOSE: A pharmaceutical composition and health functional food containing thienylbutyl isothiocyanate are provided to inhibit adipocyte differentiation. CONSTITUTION: A pharmaceutical composition for preventing and treating obesity and lipid-related metabolic disorders contains 0.1-50 wt% of thienylbutyl isothiocyanate as an active ingredient. The composition is administered in 0.01-500 mg/kg. The composition is manufactured in the form of powder, granules, tablets, capsules, suspension, emulsion, syrup, oral administration, external use formulation, suppository, or sterilized injection solution. A health functional for preventing and relieving obesity and lipid-related metabolic diseases contains the thienyl butyl isothiocyanate as an active ingredient. The health functional food is manufactured in the form of capsules, tablets, granules, powder, or beverage.