A method of producing a population of differentiated cells comprising: a)inducing differentiation in a first populationof cells by applying an inducer to said cells, b) harvesting microvesiclesproduced from first population of cells, and c) inducingdifferentiation in a second population of cells by applying said microvesiclesor a derivative thereof to said second population ofcells wherein, said first population of cells is autologous to said secondpopulation of cells and wherein the inducer applied to saidfirst population of cells is not present in said second population of cells oris only present in trace amounts. Also related methods ofproducing microvesicles, methods of medical and/or cosmetic treatment andrelated products and uses.
