A ventilation and temperature regulation structure for an animal house, comprising a house body and a temperature regulating device. The house body is provided with a temperature equalization device provided with a culture layer and a waste gas exhausting device. The temperature equalization device comprises a temperature equalization passage (20) penetrating through the culture layer. A plurality of ventilation holes (21) is formed in the side wall of the temperature equalization passage (20). The outlet end of the temperature regulating device communicates with the temperature equalization passage (20). The technical solution aims at providing a ventilation and temperature regulation structure for an animal house having good ventilation, waste reduction and temperature regulation effects.