A ventilation and temperature regulation structure for an animal house, comprising a house body and a temperature regulation device with a culture layer, the house body comprising a temperature equalization device and an exhaust gas extraction device, the temperature equalization device includes a temperature equalization passage passing through the culture layer, several ventilation holes are disposed on the side wall of the temperature equalization passage； and an outlet end of the temperature regulation device is connected with the temperature equalization passage. Such technical solution is to provide a temperature regulation structure for an animal house having good ventilation, waste reduction and good effect of temperature regulation.
