DETERMINATION METHOD FOR RADICAL-SCAVENGING ACTIVITIES OF FOODS USING 1,1-DIPHENYL-2-PICRYLHYDRAZYL, AND METHOD FOR USING OF AUTHENTIC COMPOUNDS AS ANTIOXIDANT
PURPOSE: Provided are determination method for radical-scavenging activities of foods using 1,1-diphenyl-2-picrylhydrazyl, and method for using of authentic compounds as antioxidant such as alpha terpinene, gamma terpinene, terpinolene, citronellal, citral, geraniol and nootkatone. The free radical scavenger prevents cardiovascular disease, cerebrovascular, cancer and aging by eliminating free radicals from the body. CONSTITUTION: The determination method for radical-scavenging activities of foods comprises step of: adding essential oil, authentic fragrance material, buffer solution, ethanol and emulsifier to the test sample then adding DPPH thereto; adding a standard anti-oxidant for the determination of the oxidation resistance to the above mixture and stirring it in a shaker; settling it in the dark at room temperature for 30 minutes and determining the decrease of the DPPH peak by HPLC at 517nanometer; and determining the anti-oxidancy of the sample by using the peak height of HPLC according to formula of
