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酵素液の製造方法及び糖の製造方法
专利权人:
独立行政法人産業技術総合研究所
发明人:
秀野 晃大,井上 宏之,塚原 建一郎,遠藤 貴士,澤山 茂樹
申请号:
JP2009155326
公开号:
JP5339250B2
申请日:
2009.06.30
申请国别(地区):
JP
年份:
2013
代理人:
摘要:

PROBLEM TO BE SOLVED: To provide a method for producing an enzyme liquid by which the enzyme liquid having a high property of degrading polysaccharides can easily be produced, and to provide a method for producing a saccharide using the enzyme liquid obtained by the method.

SOLUTION: The method for producing the enzyme liquid includes: a cutting step S1 of cutting lignocellulosic biomass so as to provide a maximum diameter of &le2 mm, and providing biomass particles a pulverizing step S2 of mixing the biomass particles and water so as to afford a solid fraction of 0.1-20 mass%, providing a mixed liquid, continuously pulverizing the mixed liquid under conditions of 0-50°C temperature, and converting the mixed liquid into a slurry-like carbon source and an enzyme producing step S3 of mixing the carbon source with a culture medium, and a polysaccharide-degrading enzyme producing strain, carrying out culturing, and thereby affording the enzyme liquid. The method for producing the saccharide includes using the method.

COPYRIGHT: (C)2010,JPO&INPIT

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