This invention discloses a preparing method of camellia oil. The method includes the following steps: grinding shelled camellia seeds to prepare seed powder mixing the seed powder and an ethanol aqueous solution at room temperature, and the weight ratio of the seed powder and the ethanol aqueous solution is 1:1 to 1:2 and centrifuging the mixed seed powder and the ethanol aqueous solution to obtain an oil supernatant as camellia oil.本發明係提供一種苦茶油之製備方法,包含下列步驟:將脫殼的苦茶樹種子粉碎形成種子粉末。在室溫下進行種子粉末以及乙醇水溶液的混合,且使種子粉末以及乙醇水溶液的重量比例介於1:1至1:2。以及對混合完全的種子粉末以及乙醇水溶液進行離心,於離心後取得懸浮於液體上層的油脂,而取得苦茶油。S10~S40‧‧‧步驟