Jeroen Kortsmit,Niels Jozef Bart Driessen,Marcel Cornelis Maria Rutten,Franciscus Petrus Thomas Baaijens
申请号：
US12865148
公开号：
US08609415B2
申请日:
2009.02.02
申请国别(地区):
US
年份:
2013
代理人:
摘要：
Method for manufacturing a tissue-engineered construct, such as a heart valve, comprising the steps of providing a-cell-seeded scaffold in a bioreactor chamber which bioreactor chamber is divided by the cell-seeded scaffold into a first compartment and a second compartment, subjecting the cell-seeded scaffold to a flow of nutrient medium within the bioreactor chamber for developing the cell-seeded scaffold to a tissue structure and next to the tissue construct, applying a dynamic pressure difference to the developing tissue structure by the flow of nutrient medium to induce dynamic strain on the tissue structure.
