It is aimed to provide a porous cell scaffold having a thickness of 2 mm or more and being capable of easily seeding cells in a whole small hole structure of a scaffold without applying high force to the cells and almost accurately grasping an amount of cells in the scaffold, and a production method thereof. The production method includes steps of filling a guiding solution having kinematic viscosity which is 50 to 450% of kinematic viscosity of a culture medium, in a whole continuous small hole structure of a sheet-shaped or block-shaped scaffold having the continuous small hole structure with hole diameters of 5 to 3200??m and an average hole diameter of 50 to 1500??m, a three-dimensional structure, and a thickness of 2 mm or more, supplying thereafter a culture medium with cells being suspended to an upper side of the scaffold, sucking the guiding solution from a lower side of the scaffold by low suction force, and entering thereby the culture medium with cells being suspended into the whole small hole structure of the scaffold. It is preferable that a water absorber such as a filter paper is used for sucking the guiding solution by low suction force.
