The Trustees Of The University Of Pennsylvania ;Anima Cell Metrology, Inc.
发明人:
Cooperman Barry S.,Smilansky Zeev,Goldman Yale E.,Pan Dongli
申请号：
US201715428750
公开号：
US2017153242(A1)
申请日:
2017.02.09
申请国别(地区):
美国
年份:
2017
代理人:
摘要：
Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system. The disclosed met
