НЭЙЛОР Дин Джейсон (AU),БРАУН Ричард Джеймс (AU),ХОВАРД Кристофер Брюс (AU),ДЕ БЕККЕР Кристофер Джон (AU),СТОК Джанетт Элизабет (AU),ДЖЕЙМС Эндрю Ли (AU),ЛАМБЕРТ Дэниел Скотт (AU),РЭЛСТОН Кайли Джейн
申请号：
RU2012117610/10
公开号：
RU2012117610A
申请日:
2010.10.08
申请国别(地区):
RU
年份:
2013
代理人:
摘要：
1. The isolated polypeptide of the variant chaperonin 10 (Cpn10) having at least 90% sequence identity with the wild-type Cpn10 polypeptide and containing an N-terminus extended by at least two additional amino acid residues compared to the specified wild-type polypeptide, where the polypeptide has an increased binding affinity for the pathogen-associated molecular pattern (PAMP) compared to the binding affinity of Ala-Cpn10 (SEQ ID NO: 3) with said PAMP. 2. The isolated Cpn10 variant polypeptide of claim 1, wherein the N-terminus of said variant polypeptide begins with a methionine residue. The isolated Cpn10 variant polypeptide according to claim 2, wherein said methionine residue precedes an amino acid residue selected from the group consisting of arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threon tryptophan, tyrosine and valine. 4. The isolated Cpn10 variant polypeptide of claim 3, wherein said polypeptide contains an amino acid sequence as set forth in any of SEQ ID NO: 9, 12, 15, 18, 24, 26, 29, 32, 38, 41, 44, 47, 49 , 78, 90, or 93.5. An isolated nucleic acid encoding a polypeptide according to claim 1.6. The isolated nucleic acid according to claim 5, wherein said nucleic acid contains a nucleotide sequence as set forth in any of SEQ ID NO: 10, 13, 14, 16, 17, 19, 20, 25, 27, 28, 30, 31, 33 , 34, 39, 40, 42, 43, 45, 46, 48, 50, 51, 91, 92, 94, or 95.7. The isolated Cpn10 variant polypeptide of claim 1, wherein said polypeptide contains an amino acid sequence as set forth in any of SEQ ID NO: 52, 55, 58, 60, 81, 84, 87, or 96.8. An isolated nucleic acid encoding a polypeptide according to claim 7.9. Dedicated �1. Выделенный полипептид варианта шаперонина 10 (Cpn10), имеющий по меньшей мере 90% идентичность последовательностей с полипептидом Cpn10 дикого типа и содержащий N-конец, удлиненный, по меньшей мере на два дополнительных аминокислотных остатка по сравнению
