PROBLEM TO BE SOLVED: To provide a mushroom bed cultivation method of a mushroom of a large size having an excellent shape and crunchy texture, a mushroom cutting useful for the cultivation method, a mushroom bed cultivation culture medium into which the cutting is transplanted, and a mushroom bed cultivation culture medium which is suitably used for mushroom bed cultivation.SOLUTION: A mushroom bed cultivation method of Lyophyllum shimeji includes the steps of: isolating a young fruit body 5-20 mm in length of Lyophyllum shimeji, which is differentiated from a mushroom primordium obtained by mushroom bed cultivation, from a mushroom cultivation culture medium； transplanting the isolated young fruit body to a mushroom bed of a mushroom bed cultivation culture medium over which fungal filaments of Lyophyllum shimeji are proliferated by fitting it into a hole portion formed in the bed； and growing the transplanted young fruit body into a harvestable matured fruit body.【課題】大型で優れた形態と歯応えのある食感を有するきのこの菌床栽培方法、当該栽培方法に有用なきのこのさし芽、当該さし芽が移植された菌床栽培用培養基、及び菌床栽培に好適な菌床栽培用培養基の提供。【解決手段】ホンシメジの菌床栽培方法であって、菌床栽培で得られるきのこ原基から分化した、長さが５～２０ｍｍのホンシメジの幼子実体を菌床栽培用培養基から単離する工程、単離した幼子実体を、ホンシメジ菌糸が蔓延した菌床栽培用培養基の菌床上に形成された孔部にかん合させて幼子実体を移植する工程、及び移植された幼子実体を収穫可能な成熟子実体に生育させる工程、を包含することを特徴とする方法が提供される。【選択図】なし
