To provide a treatment method of a heart for transplantation capable of preserving a heart for transplantation for a long time in an appropriate state.SOLUTION: The treatment method of a heart for transplantation according to the present invention includes: a perfusion process of perfusing a heart H for transplantation using a perfusion liquid; a cooling process of immersing the heart H for transplantation after the perfusion process in an organ preservation liquid L contained in a perfusion container 6, and immersing the container 6 containing the heart H for transplantation in a liquid cooling medium B controlled to -6°C to -10°C in a liquid tank 3 of a cooling device 1; and a reperfusion process before transplantation.SELECTED DRAWING: Figure 2【課題】移植用の心臓を長時間にわたって適切な状態で維持可能な移植用心臓の処理方法の提供。【解決手段】本発明の移植用心臓Hの処理方法は、灌流液を用いて移植用心臓Hを灌流する灌流工程と、容器体6に収容された臓器保存液Lに、灌流工程後の移植用心臓Hを浸漬し、移植用心臓Hが収容された容器体6を、冷却装置1の液槽3内で-6℃~-10℃に管理された液体の冷却溶媒Bに浸漬する冷却工程と、移植前の再灌流工程と、を含む。【選択図】図2