KIM, Jun Sic,LEE, Gun Sul,KIM, Ki-Yong,KANG, Yong,SON, Ki Hwan
申请号：
KRKR2012/008755
公开号：
WO2013/062305A1
申请日:
2012.10.24
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
Provided are a method for preparing a highly concentrated fibrinogen solution, and a method for preparing a fibrin sealant component 1 containing the highly concentrated fibrinogen solution and Factor ΧIII. More particularly, the method for preparing a highly concentrated fibrinogen solution according to the present invention can be prepared by adding amino acid or amino acid derivatives, and/or salts, etc. to a lowly concentrated fibrinogen solution separated from blood plasma and highly concentrating fibrinogen using an ultra filtration method, and a fibrin sealant component 1 in order to be used in a fibrin sealant product can be prepared by adding Factor ΧIII before or after the ultra filtration in the method for preparing the highly concentrated fibrinogen. The fibrin sealant component 1 prepared by the method according to the present invention may be cryopreserved in a liquid state, be preserved for a long time at room temperature, and be immediately applied to treatment without performing a reconstitution process. In addition, the present invention relates to a fibrin sealant product configured of the fibrin sealant component 1 and a fibrin sealant component 2 which is a solution containing thrombin and calcium chloride filled in a separate container. The fibrin sealant product according to the present invention may be provided in a vial type in which the fibrin sealant component 1 and the fibrin sealant component 2 are filled in separate vials, respectively, or a pre-filled type in which the fibrin sealant component 1 and the fibrin sealant component 2 are filled in separate syringes connected with each other to thereby be immediately used.L'invention propose un procédé de préparation d'une solution hautement concentrée en fibrinogène et un procédé de préparation d'un composant 1 de colle de fibrine contenant la solution hautement concentrée en fibrinogène et le Facteur XIII. Plus particulièrement, le procédé de préparation d'une solution hautement concentré
