KIM, JUN SIC,LEE, GUN SUL,KIM, KI-YONG,KANG, YONG,SON, KI HWAN
申请号：
CA2852019
公开号：
CA2852019C
申请日:
2012.10.24
申请国别(地区):
CA
年份:
2017
代理人:
摘要：
Provided are a method for preparing a highly concentrated fibrinogen solution, and a method for preparing a fibrin sealant component 1 containing the highly concentrated fibrinogen solution and Factor ?III. More particularly, the method for preparing a highly concentrated fibrinogen solution according to the present invention can be prepared by adding amino acid or amino acid derivatives, and/or salts, etc. to a lowly concentrated fibrinogen solution separated from blood plasma and highly concentrating fibrinogen using an ultra filtration method, and a fibrin sealant component 1 in order to be used in a fibrin sealant product can be prepared by adding Factor ?III before or after the ultra filtration in the method for preparing the highly concentrated fibrinogen. The fibrin sealant component 1 prepared by the method according to the present invention may be cryopreserved in a liquid state, be preserved for a long time at room temperature, and be immediately applied to treatment without performing a reconstitution process. In addition, the present invention relates to a fibrin sealant product configured of the fibrin sealant component 1 and a fibrin sealant component 2 which is a solution containing thrombin and calcium chloride filled in a separate container. The fibrin sealant product according to the present invention may be provided in a vial type in which the fibrin sealant component 1 and the fibrin sealant component 2 are filled in separate vials, respectively, or a pre-filled type in which the fibrin sealant component 1 and the fibrin sealant component 2 are filled in separate syringes connected with each other to thereby be immediately used.
