Rose, Scott,Behlke, Mark A.,Owczarzy, Richard,Walder, Joseph A.,Thomas, Derek M.,Marvin, Michael R.
申请号：
AU2011230496
公开号：
AU2011230496A8
申请日:
2011.03.28
申请国别(地区):
AU
年份:
2012
代理人:
摘要：
A composition, comprising a first oligonucleotide having the structure 5-YLX-L_2-Y-3 Y comprises a sequence of four or more DNA or RNA nucleotides, including a first nucleotide N having a phosphate covalently linked to L Ycomprises a sequence of four or more DNA or RNA nucleotides, including a second nucleotide Nhaving a 5 phosphate covalently linked to L Land Leach independently are a direct bond or a CC alkyl, alkynyl, alkenyl, heteroalkyl, substituted alkyl, aryl, heteroaryl, substituted aryl, cycloalkyl, alkylaryl, or alkoxyl group. X is R\ is a hydrogen or a d-Cg alkyl. M is a quencher. The first oligonucleotide is adapted to hybridize to a second oligonucleotide having the structure 3-Υ3-Υ4-5. Y3 comprises a sequence of four or more DNA or RNA nucleotides, including a third nucleotide N3. Y4 comprises a sequence of four or more DNA or RNA nucleotides, including a fourth nucleotide N4 that is directly attached to nucleotide N. If the first oligonucleotide hybridizes to the second oligonucleotide, Ni base pairs with N and N2 base pairs with N4.to form a duplex having a T that is greater than the T of a duplex formed between the second oligonucleotide and a third oligonucleotide having the structure 5-Y-Y-
