A multi-function biological sensing device is provided, the multi-function biological sensing device includes a multi-function card, a multi-function card slot, a first multi-function module and a second multi-function module. The multi-function card includes a first pin set. The multi-function card slot is configured to receive the multi-function card, and includes a second pin set. The first multi-function module is disposed on the multi-function card, and electrically connected to the first pin set. The second multi-function module is disposed on the multi-function card slot, and electrically connected to the second pin set. Then, the first multi-function module and the second multi-function module perform a electrical connection through the first pin set and the second pin set, so as to provide at least one additional function.一種多功能生物感測裝置,所述多功能生物感測裝置包括多功能卡、多功能卡插槽、第一多功能模組以及第二多功能模組。多功能卡具有第一接腳組。多功能卡插槽用以接收多功能卡,並且具有第二接腳組。第一多功能模組設置於多功能卡,並且電性連接上述第一接腳組。第二多功能模組設置於多功能卡插槽,並且電性連接上述第二接腳組。然後,第一多功能模組與第二多功能模組透過上述第一接腳與上述第二接腳組進行電性連接,以提供至少一附加功能。10‧‧‧多功能生物感測裝置11‧‧‧多功能卡111、112、121、122‧‧‧接腳組113、123‧‧‧印刷電路板12‧‧‧多功能卡插槽124‧‧‧插槽本體13‧‧‧第一多功能模組14‧‧‧第二多功能模組