Obshchestvo s ogranichennoj otvetstvennostyu "Matrifleks"
发明人:
VEREMEEV Aleksej Vladimirovich (RU),ВЕРЕМЕЕВ Алексей Владимирович (RU),KUTIKHIN Anton Gennadievich (RU),КУТИХИН Антон Геннадиевич (RU),NESTERENKO Vladimir Georgievich (RU),НЕСТЕРЕНКО Владимир Георгиев,VEREMEEV Aleksej Vladimirovich,ВЕРЕМЕЕВ Алексей Владимирович,KUTIKHIN Anton Gennadievich,КУТИХИН Антон Геннадиевич,NESTERENKO Vladimir Georgievich,НЕСТЕРЕНКО Владимир Георгиевич,BOLGARIN Roman Nikolae
申请号：
RU2017108893
公开号：
RU0002665962C1
申请日:
2017.03.17
申请国别(地区):
RU
年份:
2018
代理人:
摘要：
FIELD: medicine； biotechnology.SUBSTANCE: group of inventions relates to medicine, namely biotechnology, and can be used to produce a bioresorbable biological matrix to replace defects of bone tissue from xenogeneic or allogeneic bone tissue. Method comprises the following sequential steps: a) step of pretreating said xenogeneic or allogeneic bone tissue, including one or more cycles of its freezing at temperatures from -20 to -80 °C and thawing at temperatures from +5 to +37 °C； b) step of deep purification and extraction of the material obtained in the previous step, comprising treating the material in a solution of ionic or ionic and amphoteric detergent in a buffer solution, then, optionally, washing in a buffer solution, then further processing in a non-ionic detergent solution, followed by extraction of the material using an ultrasonic disintegrator； c) step of delipidating the material obtained in the previous step, comprising treating the material in a solution of ethanol and/or ethanol-chloroform and/or ethanol-ethyl acetate and toluene, and then treatment with sodium hydroxide； d) step of fermenting the material obtained in the previous step, including treating the material in DNAase and/or trypsin solutions； e) step of demineralizing the material obtained in the previous step, including treating the material with a solution of a strong acid followed by neutralization with sodium hydroxide； f) impregnation step comprising impregnating the material obtained in the previous step with vesicular phosphatidyl choline and/or cholesterol to which gelatin (hydrolysed collagen) and/or bone atelocollagen and/or poly (e-caprolactone) are included. Group of inventions also relates to the use of a bioresorbable biological matrix for filling bone tissue defects or as an implant in surgery.EFFECT: use of this group of inventions makes it possible to obtain a biological matrix with enhanced osteo- and biointegration, high biocompatibility and lack of immunoreactivity on t
