MICROBIOLOGY INSTITUTE OF SHAANXI;INSTITUTE OF BIOPHYSICS, CHINESE ACADEMY OF SCIENCES
发明人:
WAN, Yi,CHEN, Chang,GAO, Lei,ZHANG, Yuying,QIAO, Xinhua,WANG, Yan
申请号：
WO2016CN102973
公开号：
WO2017067516(A1)
申请日:
2016.10.22
申请国别(地区):
世界知识产权组织国际局
年份:
2017
代理人:
摘要：
Provided are a method, target and application for NO accumulation to decrease invasiveness of Pseudomonas aeruginosa (P. aeruginosa); achieving NO accumulation by means of external NO donors or disruption of internal NO metabolism in an environment where P. aeruginosa is growing or being cultured, in order to decrease production by P. aeruginosa of the invasion factor pyocyanin. Utilizing even micro-quantities of NO can significantly inhibit P. aeruginosa PCN (PCN synthesis down 82% with 60μM SNP treatment); by inhibiting NO reductase, an enzyme related to NO metabolism, PCN synthesis can be significantly inhibited (the PAO1 Δnor strain has 84% PCN reduction). Using NO donors or inhibiting enzymes involved in P. aeruginosa NO metabolism as a means of combating bacterial infectious disease does not affect the body's normal microflora like traditional antibiotics.
