CARLSON DALE,PEARCE JAMES,SINGH BIJAY,SCHOPKE CHRISTIAN,WALKER KEITH,GOCAL GREG,MC ELVER JOHN,BEETHAM PETER
申请号：
NZ60741109
公开号：
NZ607411A
申请日:
2009.09.24
申请国别(地区):
NZ
年份:
2014
代理人:
摘要：
Disclosed is a method for controlling weeds comprising: providing a seed capable of producing a Brassica plant comprising a nucleic acid molecule encoding a Brassica napus AHASL III protein comprising an alanine to threonine substitution at a position corresponding to position A122 of SEQ ID NO:23 and a serine to asparagine substitution at a position corresponding to position S653 of SEQ ID NO:23, wherein the AHASL III protein is resistant to inhibition by an imidazolinone herbicide at a synergistic level as compared to the additive levels of the imidazolinone resistance of a Brassica napus AHASL III protein having the single mutation A122T and the imidazolinone resistance of a Brassica napus AHASL III protein having the single mutation S653N； and contacting the seed with an imidazolinone herbicide, before sowing and/or after pregermination. Further disclosed are methods for identifying AHASL III nucleic acid molecules from Brassica napus containing the Asn 122 Thr and Ser 653 Asn mutation.
