CIGAN ANDREW MARK,FALCO SAVERIO CARL,LASSNER MICHAEL,LIU ZHAN BIN,SVITASHEV SERGEI
申请号：
IN201617008550
公开号：
IN201617008550A
申请日:
2016.03.11
申请国别(地区):
IN
年份:
2016
代理人:
摘要：
Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide / Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant plant cell or seed without incorporating a selectable transgene marker. Once a genomic target site is identified a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide / Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell without incorporating a selectable transgene marker.
