E. I. DU PONT DE NEMOURS AND COMPANY;PIONEER HI-BRED INTERNATIONAL, INC.
发明人:
Andrew Cigan,Saverio Carl Falco,Huirong GAO,Zhongsen Li,Zhan-Bin Liu,L. Aleksander Lyznik,Jinrui Shi,Sergei Svitashev,Joshua K. Young
申请号：
US14913614
公开号：
US20160208272A1
申请日:
2014.08.20
申请国别(地区):
US
年份:
2016
代理人:
摘要：
Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
